Method

Site-directed mutagenesis

Techniques that introduce specific nucleotide changes into a gene sequence—through directed mutagenesis, point mutations, or chemical mutagens—to probe structure-function relationships.

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Scholars using this

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CN
Crina NimigeanDistinguished Professor of Anesthesiology Research II
CN
Crina NimigeanDistinguished Professor of Anesthesiology Research II
AA
Alessio AccardiProfessor of Biochemistry and Biophysics in Anesthesiology
AA
Alessio AccardiProfessor of Biochemistry and Biophysics in Anesthesiology
DE
David EliezerProfessor
DE
David EliezerProfessor
SR
Samara Reck-PetersonChair of Biochemistry and Biophysics
SR
Samara Reck-PetersonChair of Biochemistry and Biophysics
BS
Beate SchwerProfessor of Microbiology and Immunology
BS
Beate SchwerProfessor of Microbiology and Immunology
RG
Ronald G CrystalChair of Genetic Medicine
RG
Ronald G CrystalChair of Genetic Medicine

Spotlight

Site-directed mutagenesis

AKAnant K Menon

Protein science : a publication of the Protein Society · 2026

Site-directed mutagenesis

HYHee-Seop Yoo SSSimon Scheuring CNCrina Nimigean

Nature communications · 2026

Site-directed mutagenesis

OAOmar AlvarengaGKGeorge Khelashvili AAAlessio Accardi

Nature structural & molecular biology · 2026

View all 22 publications →

Publications

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Site-directed mutagenesis

Scholars using this

Mechanism-guided mutagenesis of Rft1 to test its role as a dolichol-linked oligosaccharide scramblase in cells.

Lipid trapping slows ball-and-chain inactivation in a calcium-activated potassium channel.

Calcium dependent activation of the TMEM16F scramblase and ion channel.